Department of Biochemistry

Recent Publications

Zhang,X., Jlang, G., Cai, Y., Monkley, S.J., Critchley, D.R. and Sheetz, M.P. (2008) Talin depletion reveals independence of initial cell spreading from integrin activation and traction. Nature Cell Biol. 10: 1062-1068

Lightbody, K.L., Ilghari, D., Waters, L.C., Carey, G., Bailey, M.A., Williamson, R.A., Renshaw, P.S. and Carr, M.D. (2008) Molecular features governing the stability of functional complex formation by Mycobacterium tuberculosis CFP-10/ESAT-6 family proteins. J. Biol. Chem. in press (advance online publication 2/1/04/2008 doi/10.1074/jbc.M800123200)

Elliott, P.R., Mohammed, S., Melrose, H.J. and Moody, P.C.E.M.(2008) Expression, purification, crystallisation and preliminary X-ray analysis of a NADP-dependent Glyceraldehyde-3-phosphate Dehydrogenase from Helicobacter pylori. Acta Crystallographica F64 727-729

Lammerman, T., Bader, B.L., Monkley, S.J., Worbs, T., Wedlich-Soldner, R., Hirsch, K., Keller, M., Forster, R., Critchely, D.R., Fassler, R and Sixt M. (2008) Rapid leukocyte migration by integrin-independent flowing and squeezing. Nature 453. 51-55

Conti, F.J., Felder,A., Monkley, S., Schwander, M., Wood, M.R., Lieber, R., Critchley, D.R and Muller U (2008) Progressive myopathy and defects in the maintenance of myotendinous junctions in mice that lack talin 1 in skeletal muscle. Development 135, 2043-2053

Veverka, V., Crabbe, T., Bird, I., Lennie, G., Muskett, F.W., Taylor, R.J. and Carr, M.D. (2008) Structural characterization of the interaction of mTOR with phosphatidic acid and a novel class of inhibitor: compelling evidence for a centrol role of the FRB domain in small molecule-mediated regulation of mTOR. Oncogene 27, 585-595

Wasmuth, J., Schmid, R., Hedley, A. and Blaxter, M. (2008) On the extent and origins of genic novelty in the Phylum Nematoda. PLos Neglected Tropical Diseases, 2(7):e258

Wegener, K.L., Basran, J., Bagshaw, C.R., Campbell, I.D., Roberts, G.C.K., Critchley, D.R. and Barsukov. I. (2008) Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 sub-domain. J. Mol. Biol. 382, 112-116

Benahmed, F., Gross, I., Gaunt, S.J., Beck, F., Jehan, F., Domon-Dell, I., Kedinger, M., Freund, J-N and Duluc, I. Multiple regulatory regions control the complex expression pattern of the mouse Cdx2 homeobox gene. Gastroenterology in press

Stringer, E.M., Pritchard, C.A. and Beck, F. (2008) Cdx2 initiates histodifferentiation of the midgut endoderm. FEBS letters in press

Ansari, S., Alahyan, M., Marston, S.B. and El-Mezgueldi, M. (2008) Role of caldesmon in the Ca2+- regulation of smooth muscle thin filaments: Evidence for a co-operative switching mechanism. J. Biol. Chem. 283, 47-56

El-Mezgueldi, M. and Marston, S.B. (2008) Role of Tropomyosin in the regulation of smooth muscle. Tropomyosin Ed by P Gunning in press

Elliott, P.R., Evans, D., Greenwood, J. and Moody, P.C.E.M.(2008) Expression, purification, crystallisation and preliminary X-ray analysis of a NADP-dependent Glyceraldehyde-3-phosphate Dehydrogenase from Helicobacter pylori. Acta Crystallographica F64 723-726

Lienard, B.M.R., Garau, G., Horsfall, L., Karsisiotis, A.I., Damblon, C., Lassauz, P., Papamichael, C., Roberts, G.G.K., Galleni, M., Dideberg, O., Frere, J-M. and Schofield, C.J. (2008) Structural basis for the broad-sectrum inhibition of metallo-ß-lactomases by thiols. Org. Biomol. Chem. in press

Schmid, R and Blaxter, M. L. (2008) annot8r:GO, EC and KEGG annotation of EST datasets. BMC Bioinformatics 9, 180

O'Hare, H.M., Juillerat, A., Dianiskova, P. and Johnsson, K (2008) A split-protein for studying protein-protein interaction in mycobacteria. Journal of Microbiological Methods 73 (2), 79-84

Gingras, A. G., Basran, J., Prescott, A., Kriajevska, M., Bagshaw, C. R. and Barsukov, I. L. (2008) Crystal structure of the Ca2+-form and Ca2+-binding kinetics of metastasis-associated protein, S100A4. FEBS Lett.582, 1651-1656 .

Chalmers I.W., McArdle, A.J., Couslon,R.M.R., Wagner,M.A., Schmid, R., Hirai, H. and Hoffman, K.F. (2008) Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family. BMC Genomics 9, 89

Badyal, Sandip K., Metcalfe, Clive L., Basran, Jaswir, Efimov, Igor, Moody, Peter C E and Raven, Emma Lloyd (2008) Iron Oxidation state as a molecular trigger controlling active site structure in a heme peroxidase. Biochemistry. 47, 4403-4409

Hames, R.S., Hames, R., Prosser, S.L., Euteneuer, U., Lopes, C.A.M., Moore, W., Woodland, H., and Fry, A.M.,(2008) Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells. Experimental Cell Research.314, 574-589.

Gyimesi,M., Kintses,B., Bodor, A., Parczel, A., Fischer, S., Bagshaw, C. R. and Málnási-Csizmadia, A. ,(2008) The mechanism of the reverse recovery-step, phosphate release and actin activitation of Dictyostelium myosin II J.Biol. Chem.283, 8153-8163

El-Mezgueldi, M. and Bagshaw, C. R. (2008) The myosin family: biochemical and kinetic properties. Myosin (ed. Coluccio, L. M.) Springer .pp 55-93 in press

Prosser, S.L. and Fry, A.M. (2008) Flourescence imaging of the centrosome cycle in mammalian cells. Methods in Molecular Biology In press

Waters, L.C., Veverka, V., Böhm, M., Schmedt, T., Choong, P.T., Muskett, F.W., Klempnauer, K.-H. and Carr, M.D. (2007) Structure of the C-terminal MA-3 domain of the tumour suppressor protein Pdcd4 and characterisation of its interaction with eIF4A. Oncogene 26, 4941-4950.

Choquet, P., Calon, A., Breton, E., Beck, F., Domon-Dell, C., Freund, J-N. and Constantinesco, A. (2007) Multiple contrast X-ray micro-CT visualisation of colon malformations and tumours in situ in living mice. C.R. Biol. 330, 821-827

Calon, A., Gross, I., Lhermitte, B., Beck, F., Duclos, B., Kedinger, M., Duluc, I., Domon-Dell, C and Freund, J-N. (2007) Different effects of the Cdx1 and Cdx2 homeobox genes in a murine model of intestinal inflammation. Gut 56, 1688-1695

Lim J., Wiedemann, A., Tzircotis, G., Monkley, S.J., Critchley, D.R. and Caron, E. (2007) An essential role for talin during alpha(M)beta(2)-mediated phagocytosis. Mol Biol Cell. 18, 976-85.

Moes, M., Rodius, S., Coleman, S.J., Monkley, S.J., Goormaghtigh, E., Tremuth, L., Kox, C., van der Holst, P.P., Critchley, D.R. and Kieffer, N. (2007) The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton. J Biol Chem. 282, 17280-17288

Petrich, B.G., Marchese, P., Ruggeri, Z.M., Spiess, S., Weichert, R.A., Ye, F., Tiedt, R., Skoda, R.C., Monkley, S.J., Critchley, D.R. and Ginsberg, M.H. (2007) Talin is required for integrin-mediated platelet function in hemostasis and thrombosis. J Exp Med. 204, 3103-3111.

Nieswandt, B., Moser, M., Pleines, I,. Varga-Szabo, D., Monkley, S., Critchley, D.R. and Fässler, R. (2007) Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo. J Exp Med. 204, 3113-3118.

Gingras, A.R., Bate, N., Goult, B.T., Hazelwood, L., Canestrelli, I., Grossmann, J.G., Liu, H., Putz, N.S., Roberts, G.C., Volkmann, N., Hanein, D., Barsukov, I.L. and Critchley, D.R. (2007) The structure of the C-terminal actin-binding domain of talin. EMBO 27, 458-469.

Wang, Y., Litvinov, R.I., Chen, X., Bach, T.L., Lian, L., Petrich, B.G., Monkley, S.J., Critchley, D.R., Sasaki, T., Birnbaum, M.J., Weisel, J.W., Hartwig, J.and Abrams, C.S. (2008) Loss of PIP5KIgamma, unlike other PIP5KI isoforms, impairs the integrity of the membrane cytoskeleton in murine megakaryocytes. J Clin Invest. 118, 812-819

Sun, N., Critchley, D.R., Paulin, D., Li, Z. and Robson, R.M. (2008) Human alpha-synemin interacts directly with vinculin and metavinculin. Biochem J. 409, 657-667.

Mirza, M., Robinson, P., Kremneva, E., Copeland, O., Watkins, H., Levitsky, D., Redwood, C., El-Mezgueldi, M. and Marston, S.B.. (2007) The effect of mutations in a-tropomyosin (E40K and E54K) that cause familial dilated cardiomyopathies on the regulatory mechanism of cardiac muscle thin filaments. J. Biol Chem. 282, 13487-13497

Wu, W., Baxter, J.E., Wattam, S.L., Hayward, D.G., Fardilha, M., Knebel, A., Ford, E.M., da Cruz e Silva, E.F. and Fry, A.M. (2007) Alternative splicing controls nuclear translocation of the cell-cycle regulated Nek2 kinase. J of Biol Chem.282, 26431-26440

O'Regan, L., Blot, J., and Fry, A.M. (2007) Mitotic regulation by NIMA-related kinases. Cell Division .2, 25

Shi, X., Basran, J., Seward, H. E., Childs, W., Bagshaw, C. R. and Boxer, S. G. (2007) Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C): Quantitative Analysis of FRET in YFP Dimers. Biochemistry 46,14403-14417

Metcalfe, C.L., Macdonald, I.K., Murphy, E.J., Brown, K.A., Raven, E.L. and Moody, P.C.E (2007) The tuberculosis prodrug isoniazid bound to activating peroxidases. J. Biol Chem.283, 6193-6200

Goult, B.T., Rapley, D., Dart, C., Kitmitto, A., Grossman, G., Leyland, M.L. and Lian L-Y. (2007) Small-Angle X-ray scattering and NMR Studies of the Confromation of the PDZ Region of SAP97 and its interactions with Kir2.1. Biochemistry 46,14117-14128

Malygin, A., Parakhnevitch, N.M., Ivanov, A.V., Eperon, I.C. and Karpova, G.G.. (2007) Human ribosomal protein S13 regulates expression of its own gene at the splicing step by a feedback mechanism. Nuc. Acids Res. 35,6414-6423

Leszczyszyn, O.I., Schmid, R. and Blindauer, C.A.. (2007) Toward a property/function relationship for metallothioneins: Histidine coordination and unusual cluster composition in a zinc-metallothionein from plants. Proteins-Structure Function and Bioinformatics 68,922-935

Lamb, H. K., Thompson, P., Elliott, C., Charles, I. G., Richards, J., Lockyer, M., Watkins, N., Nichols, C., Stammers, D. K., Bagshaw, C. R., Cooper, A. and Hawkins, A. R. (2007) A dual role for two essential prokaryotic GTPases through interactions with the 30S subunit ribosomal proteins. Protein Science. in press.

Málnási-Csizmadia, A. , Tóth, J., Pearson , D. S., Hetenyi, C., Nyitray, L., Geeves, M. A., Bagshaw, C. R. and Kovács. M (2007) Selective perturbation of the myosin recovery stroke by point mutations at the base of the lever arm affects ATP hydrolysis and phosphate release J. Biol. Chem. 282 , 17658-17664. .

Howells, L.M., Mitra, A. and Manson, M.M. (2007) Comparison of oxaliplatin-and curcumin- mediated anti-proliferative effects in colorectal cell lines. Int J Cancer (In press)

Mann, C.D., Neal, C.P., Garcea, G., Manson, M.M., Dennison, A.R. and Berry, D.P.(2007) Prognostic molecular markers in hepatocellular carcinoma: a systematic review. Eur J Cancer (In press)

Efimov, I., Papadopoulou, ND., McLean, KJ., Badyal, SK., Macdonald, IK., Munro, AW., Moody, PCE and Raven, EL. (2007) The redox properties of ascorbate peroxidase. Biochemistry.46 (27), 8017-8023

Bagshaw, C. R. (2007) Myosin mechanochemistry. Structure, 15, 511-512.

Kohda, T.A., Tanaka, K., Konomi, M., Sato, M., Osumi, M., and Yamamoto, M. (2007) Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes. Genes to Cells, 12, 155-170

Mazereeuw-Hautier, J., Wilson, L.C., Mohammed S., Smallwood, D., Shackleton, S., Atherton, D.J., and Harper. J.(2007) Hutchinson-Gilford Progeria syndrome: clinical findings in three patients carrying the G608G mutation in LMNA and review of the literature.British Journal of Dermatology. (In press)

Book Chapter - Wallis, R and Lynch N.J. (2007)Biochemistry and genetics of the collectins in Collagen-Related Lectins in Innate Immunity (Dave Kilpatrick, editor) Research Signpost, Kerala, India pp33-56

Brown, K., Tompkins, E.M., Boocock, D.J., Martin, E.A., Farmer, P.B., Turteltaub, K. W., Ubick, E., Hemingwat, D., Horner-Glister, E. and White, I.N.H. (2007) Tamoxifen forms DNA adducts in human colon after administration of a single [14C]-labeled therapeutic dose.Cancer Res 67,6995-7002

Wallis, R., Dodds, A.W., Mitchell, D.A., Sim, R.B., Reid, K.B.M. and Schwaeble, W.J.. (2007) Molecular Interactions between MASP-2, C4 and C2 and their activation fragments leading to complement activation via the lectin pathway. J. Biol Chem , In press doi:10.1074/jbc.M606326200

Fajer, P.G., Gyimesi, M., Malnasi-Csizmadia, A., Bagshaw, C.R., Sen, K.I. and Song, L. (2007) Myosin cleft closure by double double electron resonance and dipolar EPR. J Phys Condens. Matter. 19 , 285208 (10pp)

Kintses, B., Gyimesi, M., Pearson, D.S., Geeves, M.A., Zeng, W., Bagshaw, C.R. and Málnási-Csizmadia, A. (2007) Reversible movement of switch 1 loop of myosin determines actin interaction. EMBO J. 26, 265-274 >

Rellos, P., Ivins, F.J., Baxter, J.E., Pike, A., Nott, T.J., Parkinson, D-M., Das, S., Howell, S., Federov, O., Shen, Q.Y., Fry, A.M., Knapp, S and Smerdon, S.J. (2007) Structure and regulation of the human Nek2 centrosomal kinase. Journal of Biological Chemistry. 282, 6833-6842

Bignell, D.R.D., Bate, N and Cundliffe, E. (2007) Regulation of tylosin production: role of a Tylp-interactive ligand. Molecular Microbiology (In press)

Alahyan, M., Webb, M., Marston, S.B. and El-Mezgueldi, M. (2006) Kinetic mechanism of caldesmon-tropomyosin inhibition of the actomyosin ATPase. J. Biool Chem. 281, 19433-19448

Willingdale, R., Jones, D.J.L., Lamb, J.H., Quinn, P., Farmer, P.B. and Ng, L.L. (2006) Searching for biomarkers of heart failure in the mass spectra of blood plasma. Proteomics, 6, 5903-14

Cooke, M.S., Singh, R., Hall, G.K., Mistry, V., Duarte, T., Farmer, P.B. and Evans, M.D. (2006) Evaluation of enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry methodology for the analysis of 8-oxo-7, 8-dihydro2'-deoxyguanosine in saliva and urine. Free Rad Biol Med, 41, 1829-1836

Ababou, A., Gautel, M. and Pfuhl, M. (2006) Disecting the N-terminal myosin binding site of human cardiac myosin binding protein C: Structure and myosin binding of domain C2. J. Biol Chem (In press)

McLuckie, K., Lamb, J., Sandhu, J., Pearson, H., Brown, K., Farmer, P.B and Jones, D (2006) Development of A Novel Site-Specific Mutagenesis Assay using MALDI-ToF MS (SSMA-MS). Nucleic Acids Res. 34, e150

Beyerbach, A., Farmer, P.B. and Sabbione, G (2006) Biomarkers for isocyanate exposure: synthesis of isocyanate DNA adducts. Chem. Res. Toxicol 19. 1611-1618

Kohda, T.A., Tanaka, K., Konomi, M., Sato, M., Osumi, M. and Yamamoto, M. (2006) Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source to drive the adaptation processes. Genes to cells. (In press)

Moiseeva, E.P., Heukers, R. and Manson, M.M. (2007) EGFR and Src are involved in indole-3-carbinol-induced death and cell cycle arrest of human breast cancer cells. Carcinogenesis. , 28, 435-445

Saunders, R.M., Holt, M.R., Jennings, L., Sutton, D.H., Barsukov, I.L., Bobkov, A., Liddington, R.C., Adamson, E.A., Dunn, G.A. and Critchley, D.R. (2006) Role of vinculin in regulating focal adhesion turnover. Eur J Cell Biol 85, 487-500

Patel, B., Gingras, A.R., Bobkov, A.A., Fujimoto, L.M., Zhang, M., Liddington, R.R., Mazzeo, D., Emsley, J., Roberts, G.C.K., Barsukov, I.L. and Critchley, D.R. (2006) The activity of the vinculin binding sites in talin is influenced by the stability of the helical bundles that make up the talin rod. J Biol Chem 281, 7458-7467.

Gingras, A.R., Ziegler, W.H., Frank, R., Barsukov, I.L., Roberts, G.C.K., Critchely, D.R. and Emsley, J. (2005) Mapping and consensus sequence identification for multiple vinculing binding sites within the talin rod. J Biol Chem 280, 37217-37224.

Fry, A.M. and Baxter, J.E. (2006) Sealed with a Kiz: how Plk1 ensures spindle pole integrity. Developmental Cell. 11, 431-432

Agostini, M., Schoenmakers, E., Mitchell, C.S., Szatmari, I., Savage, D.B., Smith, A.G., Rajanayagam, O., Luan, J., Semple, R.K., Bath, L., Zalin, A.N., Labib, M., Kumar, S., Simpson, H., Blom, D., Marais, D., Schwabe, J.W., Barroso, I., Trembath, R., Nagy, L., Wareham, N., Gurnell, M., O'Rahilly,S. and Chatterjee, V.K.K. (2006) A novel class of human PPARg mutations cause lipodystrophic insulin resistance by dominant negative inhibition via a non-DNA binding, interference mechanism. Cell Metabolism. 4, 303-311

Benoit, G., Cooney, A., Giguere, V., Ingraham, H., Lazar, M., Muscat, G., Perlmann, T., Renaud, J.P., Schwabe, J.W., Sladek, F., Tsai, M.J. and Laudet, V (2006) Orphan nuclear receptors. Pharmacological Reviews. In press

Jaaskelainen, J., Deeb, A., Schwabe, J.W., Mongan, N.P., Martin, H. and Hughes, I.A (2006) Human androgen receptor gene ligand-binding-domain mutations leading to disrupted interaction between the N- and C-terminal domains. J Mol Endocrinol 36, 361-368

Zeng, W., Seward, HE., Malnasi-Csizmadia, A., Wakelin, S., Woolley, RJ., Cheema, GS., BAsran, J., Patel, TR., Rowe, AJ., and Bagshaw, CR. (2006) Resonance Energy Transfer between Green Flourescent Protein Variants: complexities revealed with Myosin Fusion Proteins. Biochemistry. 45, 10482-10491

Cundliffe, E. (2006) Antibiotic production by actinomycetes: the Janus faces of regulation. Journal of Industrial Microbiology. 33, 500-506

Bate, N., Bignell, DRD., and Cundliffe, E. (2006) Regulation of tylosin biosynthesis involving 'SARP-helper' activity. Molecular Microbiology. 62, 148-156

Neal, CP., Garcea, G., Doucas, H., Manson, MM., Sutton, CD., Dennison, AR., and Berry, DP. (2006) Molecular prognostic markers in resectable colorectal liver metastases: A systematic review. European J Cancer. 42, 1728-1743

Doucas, H., Garcea, G., Neal, CP., Manson, MM., and Berry, DP. (2006) Chemoprevention of pancreatic cancer: a review of the molecular pathways involved and evidence for the potential for chemoprevention. Pancreatol. 6, 429-439

Yu, J., Paine, M.J.I.., Kemp, C.A., Marechal, J.-D., Ward, C.J., Brown, S., Sutcliffe, M.J., Roberts, G.C.K., Rankin, E.M., and Wolf,C.R. (2006) In silico prediction of drug binding to cytochrome P450 2D6: identification of a new metabolite of metoclopramide. Drug Metabolism & Distribution. doi: 10.1124/dmd.106.009258

Gingras, A.R., Vogel, K.-P., Steinhoff, H.-J., Ziegler, W.H., Frank, R., Patel, B., Emsley, J., Critchley, D.R., Roberts, G.C.K., and Barsukov, I.L. (2006) Structural and dynamic characterisation of a vinculin binding site in the talin rod. Biochemistry. 45, 1805 - 1817 http://pubs.acs.org/cgi-bin/article.cgi/bichaw/2006/45/i06/html/bi052136l.html

Maréchal, J.-D., Yu, J., Brown, S., Kapelioukh, I., Rankin, E.M., Wolf, C.R., Roberts, G.C.K., Paine, M.J.I., and Sutcliffe, M.J. (2006) In silico and in vitro screening for inhibition of cytochrome P450 CYP3A4 by co-medications commonly used by patients with cancer. Drug Metabolism & Distribution, 34, 534 - 538

Hoh, C., Boocock, D., Marczylo, T., Singh, R., Berry, D.P., Dennison, A.R., Hemingway, D., Miller, A., West, K., Euden, S., Garcea, G., Farmer, P.B., Steward, W.P. and Gescher, A. (2006) Piolt study of oral silibinin, a putative chemopreventive agent, in colorectal cancer patients: silibinin levels in plasma, colorectum, and liver and their pharmacodynamic consequences. Clin. Cancer Res. 12, 2944-2950

Singh, R., Gaskell, M., LePla, R.C., Kaur, B., Axim-Araghi, A., Roach, J., Koukouves, G., Souliotis, V.L., Kryptopoulos, S.A., and Farmer, P.B. (2006) Detection and quantitation of benzo[a] pyrene derived DNA adducts in mouse liver by liquid chromatography-tandem mass spectrometry: comparison with 32P-postlabeling. Chem. Res. Toxicol. 19, 868-878

Willmott, C. (2006) Assessing feedback and offering feedback on assessment (Editorial). Bioscience Education E-journal 7-0

Fry, A.M., and Yamano, H. (2006) APC/C-mediated degradation in early mitosis: how to avoid spindle assembly checkpoint control: Cell Cycle. 5, 1487-1491

Badayl, S.K., Joyce, M.G., Sharp, K.H., Seward, H.E., Mewies, M., Basran, J., Macdonald, I.K., Raven, E.L. and Moody. P.C.E. (2006) Conformal mobility in the active site of a heme peroxidase: J. Biol Chem. 281:34 24512-24520

MacDonald I.K., Badayl, S.K., Ghamsari, L. and Moody. P.C.E. (2006) The interaction of Ascorbate Peroxidase with substrates: A Mechanistic and Structural Analysis. Biochemistry. (in press bi 0606849).

Tunstall, R.G., Sharma, R., Perkins, S., Sale, S., Singh, R., Gant, T.W., Farmer, P.B., Steward, W.P. and Gescher, A. (2006) Cyclooxygenase-2 expression and oxidative DNA adducts in murine intestinal adenomas: modification by dietary curcumin and implications for clinical trials. Eur. J. Cancer. 42, 414-421

Jones, K., Garfitt, S., Emms, S., Warren, N., Cocker, J. and Farmer, P.B. (2006) Correlation of haemoglobin-acrylamide adducts with airborne exposure: An occupational survey: Toxicol, Letters. 162, 174-180.

Willmott, C. (2006) Never again shout, "that WOULD have been useful for my teaching!" at the TV. Bioscience Education E-journal 7, C1

LePla, R.C., Bowman, K.J., Farmer, P.B. and Jones, G.D. (2006) Phosphate alkylation in different DNA substrates: the role of local DNA sequence and electrophilic character in determining the nonrandom nature of phosphotriester adduct formation. Chem. Res. Toxicol. 19, 407-413.

Tompkins, E.M., Farmer, P.B., Lamb, J.H., Jukes, R., Dingley, K., Ubick, E., Turteltaub, K.W., Martin, E.A., and Brown, K. (2006)A novel 14C-postlabeling assay using accelerator mass spectrometry for the detection of O6-methyldeoxyguanosine adducts. Rapid Commun. Mass Spectrom. 20, 883-891.

Hayes, M.J., Kimata, Y., Wattam, S.L., Lindon, C., Mao, G., Yamano, H., and Fry, A.M. (2006) Early mitotic degradation of Nek2A depends on Cdc20-independent interaction with the APC/C. Nature Cell Biology. 8, 607-614

Waters, L.C.,Yue, B., Veverka, V., Renshaw, P., Bramham, J., Matsuda, S., Frenkiel, T., Kelly, G., Muskett, F.W., Carr, M.D. and Heery, D.M. (2006) Structural diversity in p160/CREB-binding protein coactivator complexes J.Biol. Chem 281, 14787-14795

Haque, F., Lloyd, D., Smallwood, D., Dent, C., Shanahan, C., Fry, A., Trembath, R., and Shackleton, S. (2006) SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton. Molecular & Cellular Biology. 26, 3738-3751.

van Nes, J., de Graaf, W., Lebrin, F., Gerhard, M., Beck, F., & Deschamps, J., (2006) The Cdx4 mutation affects axial development and reveals an essential role of Cdx genes in the ontogenesis of the placental labyrinth in mice. Development. 133, 419 - 428.

Singh, R., Farmer, P.B. (2006) Liquid chromatography-electrospray ionization-mass spectrometry: the future of DNA adduct detection. Carcinogenesis. 27: 178 -196.

Málnási-Csizmadia, A., Dickens, J. L., Zeng, W. and Bagshaw, C. R. (2005) Switch movements and the myosin crossbridge stroke. J. Muscle Res. Cell Motil. 26 , 31-37 .

Renshaw, P.S., Lightbody, K.L., Veverka, V., Muskett, F.W., Kelly, G., Frenkiel, T.A., Gordon, S.V., Hewinson, R.G., Burke, B., Norman, J., Williamson, R.A. and Carr, M.D. (2005) Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6. EMBO J. 24, 2491-2498.

Marei, A., Ghaemmaghami, A., Renshaw, P., Wiselka, M., Barer, M., Carr, M.D. and Ziegler-Heitbrock, L. (2005) Superior T cell activation by ESAT-6 as compared to the ESAT-6·CFP-10 complex. Int. Immunol. In press.

Willmott C and Bryant J (2005) Engaging with the ethical implications of science Proceedings of the Science Learning and Teaching Conference 2005, p.85-89.

Hayward, D.G. and Fry, A.M. (2006) Nek2 kinase in chromosome instability and cancer. Cancer Letters. 237, 155-166.

Fry, A.M. (2005) Nek2. AfCS- Nature Molecule Pages (doi:10.1038/mp.a001625.01).

Howells LM and Manson MM (2005) Prospects for plant-derived chemopreventive agents exhibiting multiple mechanisms of action. Curr Med Chem – Anti-cancer Agents. 5, 201-213.

McLuckie, K.I.E., Gaskell, M., Martin, E.A., Routledge, M., Crookston, Farmer, P.B. and Brown, K. (2005) Mutation spectra induced by a-acetoxytamoxifen-DNA adducts in human DNA repair proficient and deficient (Xeroderma pigmentosum complementation group A) cells. Biochem. 44, 8198-8205.

Gaskell, M., McLuckie, K.I.E. and Farmer, P.B. (2005) Genotoxicity of the benzene metabolites para-benzoquinone and hydroquinone. Chem. Biol. Interact. 153, 267-270.

Farmer, P.B., Kaur, B., Roach, J., Levy, L., Consonni, D., Bertazzi, P.A., Pesatori, A., Fustinoni, S., Buratti., M., Bonzini, M., Colombi, A., Popov, T., Cavallo, D., Desideri, A., Valerio, F., Pala, M., Bolognesi, C. and Merlo, F. The use of S-phenylmercapturic acid as a biomarker in molecular epidemiology studies of benzene. Chem. Biol. Interact. 153, 97-102.

Knudsen, L.E., Gaskell, M., Martin, E.A., Poole, J., Scheepers, P.T.J., Jensen, A., Autrup, H and Farmer, P.B. (2005) Genotoxic damage in mine workers exposed to diesel exhaust, and the effects of glutathione transferase genotypes. Mutation Res. Genetic Toxicol. Environ. Mutagen. 583, 120-132.

McAnaney, T. B., Zeng, W., Doe, C. E. F., Bhanji, N., Wakelin, S., Pearson, D. S., Abbyad, P., Shi, X., Boxer S. G. and Bagshaw, C. R. (2005) Protonation, photobleaching and photoactivation of Yellow Fluorescent Protein (YFP 10C): a unifying mechanism. Biochemistry 44, 5510-5524.

Howells, L.M., Hudson, E.A. Manson, M.M.(2005) Inhibition of PI3K/ PKB signaling does not account for indole-3-carbinol-induced apoptosis in breast or prostate tumor cells. Clin Cancer Res 11, 8521 - 8527

Neal CP, Berry DP, Doucas H, Manson MM, Steward W, Garcea G. (2005) Clinical aspects of natural anti-angiogenic drugs. Current Drug Targets (In press)

Farmer, P.B., Brown, K., Tompkins, E., Emms, V.L., Jones, D.J.L., Singh, R. and Phillips, D.H. (2005) DNA adducts: mass spectrometry methods and future prospects. Toxicol. Appl. Pharmacol. 207, S293-S301.

Marshall, K.R., Gong, M., Wodke, L., Lamb, J.H., Jones, D.J.L., Farmer, P.B., Scrutton, N.S. and Munro, A.W. (2005) The human apoptosis inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity. J. Biol. Chem. 280, 30735-30740.

Garte, S., Popov, T., Georgieva, T., Bolognesi, C., Taioli, E., Bertazzi, P.A. , Farmer, P. and Merlo, D.F. (2005) Biomarkers of exposure and effect in Bulgarian petrochemical workers exposed to benzene. Chem. Biol. Interact. 153-154, 247-251.

Fustinoni, S., Buratti, M., Campo, L., Colombi, A., Consonni, D., Pesatori, A.C., Bonzini, M., Farmer, P., Garte, S., Valerio, F., Merlo, D.F. and Bertazzi, P.A. (2005) Urinary t,t-muconic acid, S-phenylmercapturic acid and benzene as biomarkers of low benzene exposure. Chem. Biol. Interact. 153-154, 253-256.

Fustinoni, S., Consonni, D., Campo, L., Buratti., M., Colombi, A., Pesatori, A., Bonzini, M., Bertazzi, P.A., Fos, V., Garte, S., Farmer, P.B., Levy, L.S., Pala, M., Valerio, F., Fontana, F., Desideri, A. and Merlo, F. (2005) Monitoring low benzene exposure: comparative evaluation of urinary biomarkers, influence of cigarette smoking and genetic polymorphisms. Cancer Epidemiol. Biomarkers Prev. 14, 2237-2244.

Roberts, S.J. Stewart, A.J., Schmid, R., Blindauer, C.A., Bond, S.R., Sadler, P.J., and Farquharson, C. (2005) Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2. Biochim Biophys Acta. 1752, 73 - 82.

Hudson, E.A., Fox, L.H., Luckett, J.C.A., Manson, M.M. (2005) Ex vivo chemoprevention research possibilities. Env Toxicol Pharmacol. 21, 204-214.

Andreadi CK, Howells LM, Atherfold PA, and Manson MM. Involvement of Nrf2, p38, B-raf and NF- k B, but not PI3K, in induction of hemeoxygenase (HO-1) by dietary polyphenols. Mol Pharmacol. 69, 1033-140

Moiseeva, E.P., Howells, L.M., Fox, L.H., Manson, M.M. Indole-3-carbinol-induced cell death in cancer cells is exacerbated in a 3D environment. Apoptosis (In press).

Neal, C., Garcea, G., Doucas, H., Sutton, C.D., Manson, M.M., Berry, D.P. Molecular prognostic markers in resectable coloreectal liver metastases: a systematic review. Eur J Cancer. (In press).

McLaughlin, L.A., Paine, M.J.I., Kemp, C.A., Maréchal, J-D., Flanagan, J.U., Ward, C.J., Sutcliffe, M.J., Roberts, G.C.K., and Wolf, C.R. (2005) Why is quinidine an inhibitor of cytochrome P450 2D6? The role of key active site residues in quinine binding. J Biol Chem. 280, 38617 - 38624.

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Gingras, A.R., Ziegler, W.H., Frank, R., Roberts, G.C.K., Critchley, D.R., and Emsley, J. (2005) The talin rod contains multiple vinculin binding sites characterised by a series of conserved hydrophobic residues. J Biol Chem. 280, 37217 - 37224.

González-García, A., Pritchard, C.A., Paterson, H.F., Mavria, G., Stamp, G. & Marshall, C.J. (2005) RalGDS is required for tumour formation in a model of skin carcinogenesis. Cancer Cell. 7, 219-226.

Kamata T., Kang J., Lee T.H., Wojnowski L., Pritchard C.A. & Leavitt A.D. ((2005) A critical function for B-Raf at multiple stages of myelopoiesis. Blood. 106, 833-840.

Mercer K., Giblett S., Iwobi M., Aldridge V., Oakden A., Brown J., Marais R. & Pritchard, C. (2005) A-Raf and Raf-1 work together to influence transient ERK phosphorylation and G1/S cell cycle. Oncogene. 24, 5207-5217.

Mercer K., Giblett S., Green S., Da Rocha Dias S., Plumb M., Marais R. & Pritchard C. (2005) Expression of endogenous oncogenic V600E B-Raf induces proliferation and developmental defects in mice and transformation of primary fibroblasts. Cancer Research. 65, 11493-11500.

Cebulska-Wasilewska, A., Wiechec, A., Panek, A., Binkova, B., Sram, R.J., Farmer, P.B. (2005) Influence of environmental exposure to PAHs on the susceptibility of lymphocytes to DNA-damage induction and on their repair capacity. Mutation Res Genetic Toxicol Environ Mutagen 588, 73 - 81.

Jones, D.J.L., Jukes-Jones, R., Verschoyle, R.D., Farmer, P.B., and Gescher, A.J. (2005) A synthetic approach to the generation of quercetin sulfates and the detection of quercetin 3'-O-sulfate as a urinary metabolite in the rat. Bioorg Med Chem. 13, 6727 - 6731.

Woods, A.J., Kantidakis, T., Sabe, H., Critchley, D.R., and Norman, J.C. (2005) Interaction of paxillin with poly(A)-binding protein 1; a role in focal adhesion turnover and cell migration. Mol. Cell Biol. 25, 3763-3773.

de Pereda, J.M., Wegener, K., Santelli, E., Bate, N., Ginsberg, M.H., Critchley, D.R., Campbell, I.D., and Liddington, R.C. (2005). Structural bases for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions. J Biol Chem. 280, 8381-8386

Last updated: 26 September 2008
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